Publications

Current Publications on PubMed

Refereed Journal Articles:

1.Pask AJ, Calatayud NE, Shaw G, Wood WM,  Renfree MB. Oestrogen blocks the nuclear entry of SOX9 in the developing gonad of a marsupial mammal. In press, BMC Biology.

2.Frankenberg S, Pask, AJ, and Renfree, MB. The evolution of class V POU domain transcription factors in vertebrates and their characterisation in a marsupial. Dev Biol 2010 337:162-70 [Epub 19 October 2009]

3.Calatayud N, Pask AJ, Shaw G, Richings N, Osborn S, Renfree MB. Ontogeny of the oestrogen receptors ESR1 and ESR2 during gonadal development in the tammar wallaby, Macropus eugenii.Reproduction. 2010, 139; 599–611

4.Racine C, Pask AJ, Wijayanti GE, di Clemente N, Picard JY, Shaw G, Renfree MB, Josso N. Early Expression of the Androgen Receptor in the Sertoli Cells of a Marsupial Coincides with Downregulation of Anti-Müllerian Hormone at the Time of Urogenital Virilization. Sex Dev. 2009 Dec 30. [Epub ahead of print]

5.Renfree MB, Papenfuss AT, Shaw G, Pask AJ. Eggs, embryos and the evolution of imprinting: insights from the platypus genome. Reprod Fertil Dev. 2009;21(8):935-42.

6.Yu H, Pask AJ, Shaw G, Renfree MB. Comparative analysis of the mammalian WNT4 promoter. BMC Genomics. 2009 Sep 6;10:416.

7.Tsend-Ayush E, Lim SL, Pask AJ, Hamdan DD, Renfree MB, Grützner F. Characterisation of ATRX, DMRT1, DMRT7 and WT1 in the platypus (Ornithorhynchus anatinus). Reprod Fertil Dev. 2009;21(8):985-91.

8.Renfree MB, Hore TA, Shaw G, Marshall Graves JA, Pask AJ. Evolution of Genomic Imprinting: Insights from Marsupials and Monotremes. Annu Rev Genomics Hum Genet. 2009 Jul 16. [Epub ahead of print]

9.Wilson JD, Renfree MB, Auchus RJ, Pask AJ, Shaw G. Formation of 5alpha-reduced androgens in the testes and urogenital tract of the grey short-tailed opossum, Monodelphis domestica. Reprod Fertil Dev. 2009;21(5):649-54.

10.Tang P, Argentaro A, Pask AJ, O’Donnell L, Marshall-Graves J, Familari M, Harley VR. Localisation of the Chromatin Remodelling Protein, ATRX in the Adult Testis. J Reprod Dev. 2009 May 15. [Epub ahead of print]

11.Hu Y, Yu H, Pask AJ, O’Brien DA, Shaw G, Renfree MB. A-kinase anchoring protein 4 has a conserved role in mammalian spermatogenesis. Reproduction. 2009 Apr; 137(4):645-53.

12.Pask AJ, Papenfuss AT, Ager EI, McColl KA, Speed TP, Renfree MB. Analysis of the platypus genome suggests a transposon origin for mammalian imprinting. Genome Biol. 2009;10(1):R1.

13.Renfree, MB, Ager, EA, Shaw, G and Pask, AJ  Genomic imprinting in marsupial placentation. Reproduction 2008; 136:523-531

14.Deakin JE, Koina E, Waters PD, Doherty R, Patel VS, Delbridge ML, Dobson B, Fong J, Hu Y, van den Hurk C, Pask AJ, Shaw G, Smith C, Thompson K, Wakefield MJ, Yu H, Renfree MB, Graves JA. Physical map of two tammar wallaby chromosomes: a strategy for mapping in non-model mammals. Chromosome Res. 2008;16(8):1159-75.

15.Menzies BR, Shaw G, Fletcher TP, Pask AJ, Renfree MB. Exon 3 of the growth hormone receptor (GH-R) is specific to eutherian mammals. Mol Cell Endocrinol. [Epub ahead of print] 2008 Jul 25

16.Edwards CA, Mungall AJ, Matthews L, Ryder E, Gray DJ, Pask AJ, Shaw G, Graves JA, Rogers J; SAVOIR consortium, Dunham I, Renfree MB, Ferguson-Smith AC. The evolution of the DLK1-DIO3 imprinted domain in mammals. PLoS Biol. 6(6):e135. 2008

17.Smits G, Mungall AJ, Griffiths-Jones S, Smith P, Beury D, Matthews L, Rogers J, Pask AJ, Shaw G, VandeBerg JL, McCarrey JR; SAVOIR Consortium, Renfree MB, Reik W, Dunham I.Conservation of the H19 noncoding RNA and H19-IGF2 imprinting mechanism in therians. Nat Genet. 40(8):971-6. 2008

18.Pask AJ, Behringer RR, Renfree MB. Resurrection of DNA function in vivo from an extinct genome. PLoS ONE. 3(5):e2240. 2008

19.Ager EI, Pask AJ, Gehring HM, Shaw G, Renfree MB. Evolution of the CDKN1C-KCNQ1 imprinted domain. BMC Evol Biol. 8:163. 2008

20.Pask AJ, McInnes KJ, Webb DR, Short RV. Topical oestrogen keratinises the human foreskin and may help prevent HIV infection. PLoS ONE. 3(6):e2308. 2008

21.Warren WC, Hillier LW, Marshall Graves JA, Birney E, Ponting CP, Grützner F, Belov K, Miller W, Clarke L, Chinwalla AT, Yang SP, Heger A, Locke DP, Miethke P, Waters PD, Veyrunes F, Fulton L, Fulton B, Graves T, Wallis J, Puente XS, López-Otín C, Ordóñez GR, Eichler EE, Chen L, Cheng Z, Deakin JE, Alsop A, Thompson K, Kirby P, Papenfuss AT, Wakefield MJ, Olender T, Lancet D, Huttley GA, Smit AF, Pask AJ, Temple-Smith P, Batzer MA, Walker JA, Konkel MK, Harris RS, Whittington CM, Wong ES, Gemmell NJ, Buschiazzo E, Vargas Jentzsch IM, Merkel A, Schmitz J, Zemann A, Churakov G, Kriegs JO, Brosius J, Murchison EP, Sachidanandam R, Smith C, Hannon GJ, Tsend-Ayush E, McMillan D, Attenborough R, Rens W, Ferguson-Smith M, Lefèvre CM, Sharp JA, Nicholas KR, Ray DA, Kube M, Reinhardt R, Pringle TH, Taylor J, Jones RC, Nixon B, Dacheux JL, Niwa H, Sekita Y, Huang X, Stark A, Kheradpour P, Kellis M, Flicek P, Chen Y, Webber C, Hardison R, Nelson J, Hallsworth-Pepin K, Delehaunty K, Markovic C, Minx P, Feng Y, Kremitzki C, Mitreva M, Glasscock J, Wylie T, Wohldmann P, Thiru P, Nhan MN, Pohl CS, Smith SM, Hou S, Renfree MB, Mardis ER, Wilson RK. Genome analysis of the platypus reveals unique signatures of evolution. Nature. 453(7192):175-83. 2008 Cover Article

22.Wallis MC, Waters PD, Delbridge ML, Kirby PJ, Pask AJ, Grützner F, Rens W, Ferguson-Smith MA, Graves JA.Sex determination in platypus and echidna: autosomal location of SOX3 confirms the absence of SRY from monotremes. Chromosome Res. 15(8):949-5. 2007

23.Ager EI, Pask AJ, Shaw G, Renfree MB. Expression and protein localisation of IGF2 in the marsupial placenta. BMC Dev Biol. 8:17-. 2008.

24.Pask A.J., J.Z. Paplinska, G. Shaw, J.A.M. Graves, M.B. Renfree. Marsupial WT1 Has a Novel Isoform and Is Expressed in Both Somatic and Germ Cells in the Developing Ovary and Testis.  Sexual Development. Vol. 1, No. 3, 2007

25.Ager E, Suzuki S, Pask A.J, Shaw G, Ishino F, Renfree MB. Insulin is imprinted in the placenta of the marsupial, Macropus eugenii. Dev Biol. 2007 [Epub ahead of print]

26.Pask, AJ. Walis, M. Ezaz, T. (2006) Conference Report: The forth international symposium on vertebrate sex determination. BioEssays 28(11):1144-5

27.Suzuki S, Ono R, Narita T, Pask AJ, Shaw G, Wang C, Kohda T, Alsop AE, Marshall Graves JA, Kohara Y, Ishino F, Renfree MB, Kaneko-Ishino T. Retrotransposon silencing by DNA methylation can drive mammalian genomic imprinting. PLoS Genet. 3(4):e55.2007 Cover Article

28.Wallis MC, Delbridge ML, Pask AJ, Alsop AE, Grutzner F, O’Brien PC, Rens W, Ferguson-Smith MA, Graves JA. (2007) Mapping platypus SOX genes; autosomal location of SOX9 excludes it from sex determining role. Cytogenet Genome Res. 116(3): 232-4.

29.Yu H, Pask AJ, Shaw G, Renfree MB. (2006) Differential expression of WNT4 in testicular and ovarian development in a marsupial. BMC Dev Biol 6:44.

30.Renfree, MB, Pask, AJ and Shaw, G Sexual development in the marsupial male. Aust. J. Zool. 54: 151-158.

31.Suzuki S, Renfree MB, Pask AJ, Shaw G, Kobayashi S, Kohda T, Kaneko-Ishino T, Ishino F. (2005) Genomic imprinting of IGF2, p57(KIP2) and PEG1/MEST in a marsupial, the tammar wallaby. Mech Dev. 122(2):213-22.

32.Park DJ, Pask AJ, Huynh K, Harley VR, Renfree MB, Graves JA (2005) Characterisation of the marsupial-specific ATRY gene: implications for the evolution of male-specific function.Gene. 362:29-36.

33.Rapkins R.W., Hore, T., Smithwick, M., Ager, E., Pask, A.J., Renfree, M.B., Kohn, M., Hameister, H., Nicholls, R.D., Deakin, J.D. Graves, J.A.M. The Prader-Willi/Angelman imprinted domain was assembled recently from non-imprinted components. PLoS Genetics 2 (10) e182, 2006.

34.Pask, AJ, Kiaser, U., Hirou, H., Dalinger, A., Stockton, D., Justice, M., Behringer, R.R. Novel mouse model of Idiopathic Hypogonadotrophic Hypogonadism, caused by an ENU induced mutation within the Gonadotropin Releasing Hormone Receptor. Molecular Endocrinology 2005; 19(4): 972-81 Cover Article

35.El-Mogharbel N, Deakin J, Tsend-Ayush E, Pask AJ, Graves JA. Assignment of the DMRT1 gene to tammar wallaby chromosome 3p by fluorescence in situ hybridisation. Cytogenet Genome Res. 2005; 108(4):362.

36.Carvalho-Silva, DR, O’Neill, RJW, Brown, JD, Huyn, K, Waters, PJ, Pask, AJ, Delbridge, ML, Graves, JMG.Molecular characterization and evolution of X and Y-borne ATRX homologues in American marsupials. Chromosome Research 2004; 12:795–804.

37.Park DJ, Pask AJ, Huynh K, Renfree MB, Harley VR, Graves JA. Comparative analysis of ATRX, a chromatin remodelling protein. Gene. 2004; 339:39-48.

38.Pask, A.J., Whitworth, D.J., Mao, C-A., Wei, K-J., Sankovic, N., Graves, J.A.M., Shaw, G., Renfree, M.B., Behringer, R.R. Marsupial anti-Müllerian hormone  gene structure, regulatory elements and expression. Biology of Reproduction, 2004; 70(1):160-7.

39.Pask, AJ, Behringer, R.R., Renfree, M.B. Expression of DMRT1 in the mammalian ovary and testis – from marsupials to mice. Cytogenet. Genome Res. 2003; 101(3-4):229-36.

40.Park DJ, Pask AJ, Renfree MB, Graves JA. 3′ RACE walking along a large cDNA employing tiered suppression PCR. Biotechniques. 2003; 34:750-2, 754-6.

41.Mattiske D, Pask AJ, Shaw JM, Shaw G. Structure and expression of the follicle-stimulating hormone receptor gene in a marsupial, Macropus eugenii. Mol Reprod Dev. 2002; 63(1):24-31.

42.Pask AJ, Harry JL, Graves JA, O’Neill RJ, Layfield SL, Shaw G, Renfree MB. SOX9 has both conserved and novel roles in marsupial sexual differentiation. Genesis. 2002; 33(3):131-9.

43.Renfree MB, Pask AJ, Shaw G. Sex down under: the differentiation of sexual dimorphisms during marsupial development. Reprod Fertil Dev. 2001; 13(7-8):679-90.

44.Pask AJ, Renfree MB. Sex determining genes and sexual differentiation in a marsupial. J Exp Zool. 2001; 290(6): 586-96.

45.Pask AJ, Whitworth DJ, Shaw G, Marshall Graves JA, Behringer RR, Renfree MB. Characterization of steroidogenic factor 1 during sexual differentiation in a marsupial. Gene. 2001; 277(1-2):209-19.

46.Pask AJ, Renfree MB, Marshall Graves JA. The human sex-reversing ATRX gene has a homologue on the marsupial Y chromosome, ATRY: implications for the evolution of mammalian sex determination. Proc Natl Acad Sci U S A. 2000; 97(24):13198-202.

47.Pask AJ, Harry JL, Renfree MB, Marshall Graves JA. Absence of SOX3 in the developing marsupial gonad is not consistent with a conserved role in mammalian sex determination. Genesis. 2000; 27(4):145-52.

48.Pask AJ, Graves JA. Sex chromosomes and sex-determining genes: insights from marsupials and monotremes. Cell Mol Life Sci. 1999; 55(6-7):864-75.

49.Pask AJ, Toder R, Wilcox SA, Camerino G, Graves JA. The candidate sex-reversing DAX1 gene is autosomal in marsupials: implications for the evolution of sex determination in mammals. Genomics. 1997; 41(3):422-6.

50.Graves JAM, Cook SI, Glas R, O’Neill RWO, Pask AJ, Delbridge ML (1997) Genes involved in male reproduction as targets for biocontrol of the brushtailed possum. Biological control of Possums, Royal Society of New Zealand, Miscellaneous series 45.

Book Chapters:

1.Pask A., and Graves JAM (2001) Sex chromosomes and sex determining genes: insights from marsupials and monotremes. In: Genes and Mechanisms in Vertebrate Sex Determination (EXS), Eds: Scherer, G & Schmid, M. Birkhauser Verlag, Basel, Switzerland. EXS. pp71-95.

2.Pask AJ & Renfree, MB  (2009) Molecular Regulation of Marsupial Reproduction and Development. Ed J. Deakin, P Waters and JAM Graves Springer  New York London Paris (In press November 2009)

3.Hore, TA, Renfree, MB, Pask, AJ and Graves, JAM (2009) Genomic imprinting 2009 In Marsupial Genetics and Genomics Ed J. Deakin, P Waters and JAM Graves Springer  New York London Paris (In press November 2009)

4.Renfree MB and Pask, AJ (2009) Reproductive and developmental manipulation of the marsupial, the tammar wallaby Macropus eugenii. In Vertebrate Embryogenesis: Methods and Protocols  Human Press, Springer Ed F Peligrini, Methods in Molecular Biology Series Editor J Walker ( in press, December 2009)

Published Abstracts:

1.Hallamore S, Yow M, Webb D, Pask A, Bolton D. Decreased HIV acquisition in circumcised men – the role of the remnant foreskin. J Urol, suppl. 2009: 181, 4, abstract 173.

2.Graves JAM, Delbridge ML, Pask A (1998) X-Y homology and the evolution of sex determining and spermatogenesis genes on the Y chromosome. American Journal of Human Genetics 63. A50. (IF=11.6) published abstract

Reviews of my work:

In addition to my publications, my work in conjunction with the Baylor College of Medicine was reviewed in a Nature news article: The Real Deal (2002 – Nature News article) Nature 420: 456-457. (IF=30.1).

Articles under review:

Invited Seminars

Patterning of the mouse phallus, Kumomoto University, Department of Developmental Biology, Feburary 2008

Marsupial genome evolution, Genome Core Technology Facilities, RIKEN Genomic Sciences Center Tokyo Japan. February 2006

Imprinting in the marsupial placenta, Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, Japan. February 2006

Development of the mouse phallus. Department of Anatomy and Cell Biology. The University of Melbourne. September 2005

Two new medical models from mouse mutagenesis screens. Department of Molecular Biology, The University of Connecticut. October 2004.

Comparative genomics to define ovary development. Department of molecular genetics, MD Anderson cancer centre. October 2004.

Novel mouse model of Idiopathic Hypogonadotrophic Hypogonadism, caused by an ENU induced mutation within the Gonadotropin Releasing Hormone Receptor. Combio, Perth. September 2004. Invited speaker and session Chair. September 2004.

GnRHr Mutant mouse. Mouse Rounds. Baylor college of Medicine & Texas Children’s Hospital. Texas Medical centre 2003.

Marsupials ATRX and Sex. MRC Haematology Unit. Oxford University. Oxford, England. September 2002

Wee Willie – ENU induced micro penis mouse. Dept of Molecular and cellular Biology. Baylor College of Medicine, Texas Medial centre. December 2002

A hop down the sex determining pathway with marsupials. Department of Molecular Biology, The University of Connecticut. September 2000.

Molecular mechanisms in marsupial sex determination. South-western Medical Centre, The University of Texas, Dallas. October 2000.

The role of Sox genes in Marsupial Sex determination. Department of Physiological Science, The University of California, Los Angeles. October 2000.

Conference Presentations

Pask A.J. Paplinska, J.Z. Shaw, G. Renfree, M.B, Marsupial WT1 has a Novel Isoform and is Expressed in both Somatic and Germ Cells in the Developing Ovary and Testis. Society for Reproductive Biology, Christchurch, New Zealand, Sept 2007. Oral Presentation

Menzies, B. Pask, A.J. Fletcher, T. Shaw, G. Renfree, M. Age and sex specific regulation of the growth axis during development in the tammar wallaby: A model to study mammalian fetal growth and development. Society for Reproductive Biology, Christchurch, New Zealand, Sept 2007. Oral Presentation

Chung, J. Pask, A.J. Shaw, G. Renfree, M.B. Patterning factors in the developing gonad of the marsupial. Society for Reproductive Biology, Christchurch, New Zealand, Sept 2007. Oral Presentation

Calatayud, N. Pask, A.J. Shaw, G. Renfree. M.B. The effects of oestrogen on sexual differentiation in a marsupial, Macropus eugenii. Society for Reproductive Biology, Christchurch, New Zealand, Sept 2007. Oral Presentation

Helen Gehring Pask, A.J. Shaw, G. Renfree. The expression of steroid 5 -reductases in reproductive tissues during virilisation in the tammar wallaby, Macropus Eugenii. Society for Reproductive Biology, Christchurch, New Zealand, Sept 2007. Oral Presentation

Ager E.I.,  Pask A.J.,  Shaw G. and Renfree M.B. (2006) The role of imprinted genes in the yolk sac placenta of a marsupial, macropus eugenii. Genomic imprinting workshop, Tokyo, Nov 2006. Poster presentation

Pask A.J., Calatayud N.E.,  Renfree M.B. and Shaw G. (2006) Estrogen induced mammlian sex reversal. Forth Symposium of Vertebrate Sex determination, Kona, Hawaii. Oral Presentation

Pask, A.J., Kiaser, U., Hirou, H., Dalinger, A., Stockton, D., Justice, M., Behringer, R.R. (2004) Novel mouse model of Idiopathic Hypogonadotrophic Hypogonadism, caused by an ENU induced mutation within the Gonadotropin Releasing Hormone Receptor. Combio, Perth. Oral presentation and session chair.

Pask, A.J., Behringer, R.R. (2004) A mutation within mouse GnRHR – a novel model for Hypogonadatrophic Hypogonadism. Society of Developmental Biology, Calgary, Canada. Poster presentation.

Calatayud N.E.,  Pask A.J.,  Renfree M.B. and Shaw G. (2004) Characterisation and cloning of oestrogen receptors alpha and beta in the tammar wallaby, macropus eugenii. Combio, Perth. Poster presentation.

Ager E.I.,  Pask A.J.,  Shaw G. and Renfree M.B. (2004) IGF2 and IGF2r in the yolk sac placenta of a marsupial, macropus eugenii. Combio, Perth. Poster presentation.

Pask, A.J.., Overbeek, P., and Behringer, R.R. (2003) A mouse model for hypospadias. Society of Developmental Biology, Boston, USA. Poster presentation

Pask, A.J.., Overbeek, P., and Behringer, R.R. (2003) Mouse models of Penis Disorders – A new mouse model for Hypospadias. Third Symposium of Vertebrate Sex determination, Kona, Hawaii. Oral Presentation

Pask, A.J., Renfree, M.B. and Graves, J.A.M. (2001) Marsupial ATRY; Unlocking the secrets of ATRX. Australian Society of Reproductive Biology, Canberra, Australia. Junior Scientist Award Finalist. Oral presentation.

Pask, A.J., Renfree, M.B., O’Neill, R.J.W., Layfield, S.L., Graves, J.A.M. and Harry, J.L. (2000) The role of SOX in Sex. Australian Society of Reproductive Biology, Canberra, Australia. Junior Scientist Award Finalist. Oral presentation.

Pask, A.J., Renfree, M.B. and Graves, J.A.M. (2000) The evolution of mammalian sex determination. The second international Symposium on Vertebrate Sex Determination, Wakiki, Hawaii.Oral presentation.

Pask, A.J., Renfree, M.B. and Graves, J.A.M. (2000) The genetics of marsupial sex determination. Sex determination satellite conference, University of Melbourne, Australia. Oral presentation.

Pask, A.J., Renfree, M.B. and Graves, J.A.M. (2000) The ATRX gene maps to both the marsupial X and Y chromosome. The Genome Conference, Lorne, Australia. Poster.

Pask, A.J., Renfree, M.B., O’Neill, R.J.W., Layfield, S.L., Graves, J.A.M. and Harry, J.L. (2000) Of SOX and Sex. The Genome Conference, Lorne, Australia. Poster.

Pask, A.J. and Graves, JAM. (1998) A walk down the sex determining pathway with marsupials and monotremes. The Genome Conference, Lorne, Australia. Poster.

Pask, A.J. and Graves, JAM. (1997) The characterisation of the genes involved in sex determination from the marsupial Tammar wallaby. Genetics Society of Australia, Melbourne, Australia. Poster.

Pask, A., Wilcox, S., Toder, R. and Graves, JAM. (1996) The DAX-1 gene is autosomal in marsupials – Implications for the evolution of sex determination. The Genome Conference, Lorne, Australia. Poster.

Peer Review

I am on the editorial board of Reproduction Fertility and Development. I regularly review papers for Genesis and Developmental Dynamics. On average I review one paper per month.